Chapter 363

"Of course, Zheng, that's the lease agreement signed by Celia and the Logistics Department of MIT. As long as you pay the rent on time and don't do illegal research, you can continue to rent -"

Aurora's voice came without hesitation. Zheng Jianguo was relieved. He was not interested in continuing his research in Thomas laboratory. This does not mean that he did not know that this field is the hottest spot in the world, but because there will be no progress in the development of this technology in his memory for at least 30 years.

Therefore, When MIT cancels the previous employment agreement, Zheng Jianguo just needs to remove the title of associate professor from his business card and take back his due weekend and weekends. Anyway, the discovery of graphene and the discovery of quantum Hall effect can't run away. If he appears in textbooks in the future, it can't be erased by anyone.

In addition, Zheng Jianguo has poked out the HIV virus. In the future, his focus will only be more on the Massachusetts General Hospital. Then the rest is Celia's laboratory. After hearing Aurora's official notice, Zheng Jianguo found that if the laboratory is taken back, it will bring him no small trouble.

Qian Yunjia, who just came here, doesn't know what he thinks. Tao Ye, Yi Jinzhi and Wei Yongqiang, led by Ye minde, may have to return home in advance, because their visas are all applied for in the name of this laboratory. If the laboratory is gone, it will certainly attract the attention of the Immigration Bureau.

Although Zheng Jianguo can find someone to accommodate, no one likes to make trouble for himself.

Fortunately, MIT has no plan to take back the laboratory, and Zheng Jianguo's biggest worry is gone. He answered the question, "let's just cancel the agreement."

"OK, I'll arrange the legal department to call you the compensation after going through the formalities."

Aurora's voice disappeared on the phone. Zheng Jianguo didn't expect that there was still compensation. At this time, Celia's voice came from the microphone and said, "boss, Professor Ye said that Dr. Qian has agreed to accept the invitation of the laboratory --"

"OK, I'll come after work -"

Zheng Jianguo was not surprised that Qian Yunjia could accept the invitation. Since he began to pay attention to polymerase chain reaction at the beginning of the year, he has been collecting the latest progress on this thing.

The reason why it is the latest development is that Zheng Jianguo already knows that as early as 1971, Krapp, a disciple of Indian Nobel laureate Krana, germinated the idea of studying polymerase chain reaction technology.

In the same year, krepp published this idea in the Journal of molecular biology: "if a double stranded molecule wants to obtain two identical structures and each contains all the base lengths of the template chain including primers, it needs to add DNA polymerase to complete such a repair and replication process.

Finally, an original double stranded molecule produces two identical double stranded molecules, which need to be repeated continuously throughout the cycle, and fresh polymerase should be added every time. "

Krepp's idea is beautiful. The basic process of denaturation annealing extension described in his article is the basic principle of PCR technology, and the reason why it is the basic principle is the lack of the most critical mechanical automation.

As ye minde got this article, he quickly completed the repeated test according to the methods in the article. Although there are some differences in the results, after all, the purpose of the test is to verify whether the technical means are effective, not whether the results are correct.

As a result, ye minde only said that the repeated experiment was completed. When this sentence fell to Zheng Jianguo's ears at that time, he thought the experiment was relatively simple.

It was not until he chatted with Tao Ye a few days ago that Zheng Jianguo knew that the experiment was simple to say and would kill the researcher: "the three steps of denaturation annealing extension are to ask the researcher to carry a small basket containing test tubes and soak it in three water baths for more than ten seconds to a few seconds and then to dozens of seconds, which is to complete a cycle --"

Considering the safety factors, Zheng Jianguo did not dare to say that his purpose of studying this was to carry out the research on mechanical automation after finding the high-temperature resistant polymerase.

Of course, Zheng Jianguo knew from then on that this was the general treatment for migrant scientific research workers, and it was also the main reason why krep wanted to engage in PCR technology in 1971. Unfortunately, no one had done it in 1980.

The reason why no one can figure it out is that Zheng Jianguo also knows the reason from Tao Ye: "DNA polymerase and modified nucleic acid that can terminate the extension of reaction primers can be used in DNA sequencing experiments. This DNA hybridization detection can be used for semi quantitative detection of target DNA fragments. It is this method that the teacher completed the detection of repeated test results -"

"Strand end termination DNA sequencing!"

This is a technical invention that Zheng Jianguo is familiar with. This does not mean that he knows more about gene technology, but because the "Sanger detection method" invented by Sanger, a Nobel laureate, is the most popular innovative invention this year.

As a man who aims to win the Nobel Prize, the biggest difference between Zheng Jianguo and other researchers is that he will combine the memory of his previous life to analyze the inventions and discoveries that may be his opponent. The sequencing method invented by the Nobel Prize winner is the Mount Everest mountain that he feels invincible: "this is the key to the door of PCR technology!"

With the sequencing method, we can study the DNA fragments we are interested in at will. As long as we denature, anneal and extend dozens of times according to krepp's method, we can use this method to detect and do what we want to do.

In Zheng Jianguo's opinion, the reason why Sanger detection method is so important is that when we repeat the krep test, there will be unpredictable consequences due to different polymerase activities, which will interfere with the accuracy of the test results.

This is also the reason why Qian Yunjia resolutely gave up her original job after being invited. Of course, there are the treatment conditions offered by Zheng Jianguo: "including food and housing, 2600 US dollars after tax -"

At the bridge rice noodle shop, Zheng Jianguo invited everyone in the laboratory to dinner. On his table sat Celia Ye minde and Qian Yunjia. The others sat at four full tables in addition to two who had appointments.

Just after Zheng Jianguo said his offer, Qian Yunjia asked, "this treatment is quite good, but I want to ask if there are other positions in biology in your laboratory - my husband teaches at Montana State University. I think if there is a position in the laboratory, I can recommend him to try."

"What is your husband's research field?"

Zheng Jianguo didn't expect her to ask this question, but think that this big sister made a high-temperature polymerase four years ago. Now she will start a family four years after graduation.

Of course, this is not a big problem for Zheng Jianguo. It depends on whether he wants to add: "now there is another project in the laboratory to study the effect of nitroglycerin on blood vessels -"

"My husband studied neurobiology when he was a student. He has a master's degree in neurobiology from Montana State University. Do you have any suitable positions in that project?"

Qian Yunjia's melon seed face showed concern. He said that Zheng Jianguo's eyes stopped on the cross hanging around her neck, looked at Professor Ye minde with a smile, and then turned back and said, "there is still a lack of experimental assistant in that project, and the salary is similar to you. In addition, I know that MIT is preparing to build a bioengineering major, You can also let your husband try -- "

"OK, thank you! My lord -- "

Qian Yunjia nodded slowly. After showing piety on the melon seed face, she said that Celia's eyes quickly fell on Zheng Jianguo's face. After looking at the wonderful look on her boss's face, she looked at Ye minde, and listened to Zheng Jianguo's dry smile and said in two voices: "well, for you, I want to thank God."

Since he had read her resume before, Zheng Jianguo was already familiar with the performance of these people with devout beliefs. Then he greeted everyone to dinner. First, some people left at the next tables one after another. Finally, when Celia left with Qian Yunjia, ye minde smiled and looked at the next table of taoye and said, "I'm afraid you're not full?"

"If you don't have enough to eat, let the boss continue to work -"

Zheng Jianguo said with a smile and called the boss. After ordering four rice noodles, he watched the boss leave. Unexpectedly, ye minde said, "Jianguo, if you bring both of them in, you're not afraid that the man is an underground worker?"

"At present, the treatment on the other side of the sea is mainly Huairou. It doesn't matter whether it doesn't matter. I won't let them work in China. Besides, Qian Yunjia is more useful for our project.

In fact, I was going to ask her husband to help with you earlier. That's why I thought of this. Don't tie you to the other side of the sea, but that's why I arranged for them to be separated -- "

Zheng Jianguo clearly remembers what the two sides of the Strait will be like in the future. However, at present, he doesn't pay much attention to this project. It can be seen from MIT's attitude towards this laboratory, that is, he doesn't believe he can study anything from the effect of nitroglycerin on blood vessels. As for the research on polymerase, he won't worry about it before it starts.

"The four of us are useless and not celebrities. It's you. Don't let anyone get you over there -"

Ye minde looked at the boss and brought the rice noodles with a plate. He saw that Zheng Jianguo also looked at the boss to put down the rice noodles and said, "boss, how did your waiter do it in person?"

"I found my mother-in-law's house. The temporary one hasn't come yet. Jianguo, you eat first. We'll talk again when we're free. I have to clean the table -"

The boss who put down the rice noodles said angrily and left. Zheng Jianguo nodded without saying anything, but looked at Ye minde nearby: "it's no use getting me over. My parents are in China. Besides, they don't dare to start at this time.

Now the United States has to get rid of its relationship with them. If they come out again at this time, I'm afraid the friends will be more than frightened. Do you want to stop eating? "

"I don't want it. What you eat is -"

Looking at Zheng Jianguo's searching eyes, ye minde pushed the rice noodles in front of him, and then glanced at the fast eating taoye people on the table next to him. Just after he sighed and snored and endured a few hot bites, he said, "dig out the factors over there, you also have to pay attention to your wealth. When you said Dr. Qian's treatment earlier, I saw that someone who ate looked at you, Besides, you're dressed like a Sao bag -- "

"I've been paying attention to this. But when you say so, I feel you have to take care of Tao Ye and them. When the subsidy is paid, you'd better put it away for them. Don't go away for cheap. The public security situation in Boston is relatively good on the whole -"

Hearing Ye minde's reminder, Zheng Jianguo thought of something. He looked at several people on the table next to him. The highest salary in the laboratory before him was Celia, who was almost full-time. After tax payment, she was less than two thousand dollars a month. Others were part-time, which looked like six, seven, eight hundred after tax payment.

But ye minde and his four friends had to stay full-time for six months. When Zheng Jianguo gave subsidies to several people, he gave them $1800 according to the average standard of the United States of America. As for ye minde, it was $2600 and Celia's $2000. The old man didn't say anything when he knew.

Because the old man was 50000 yuan two years ago. After paying 20000 party dues, there are still more than 30000 yuan left. Among them, the cost of Zheng Jianguo's reselling movie tickets and the material cost of finding out Helicobacter pylori are all from the old man.

2000 U.S. dollars can be exchanged for 3000 yuan. In six months of six months, it will be 12000 U.S. dollars and 18000 yuan. However, it is exchanged according to the official quotation.

Only Zheng Jianguo knows that the $12000 can be exchanged for 100000 yuan at most in the hands of some people outside the capital Republic bank.

Of course, the old man disdains to change. Just as he won't discuss his salary with Zheng Jianguo now, ye minde is not bad for money.

Until Zheng Jianguo's voice fell, ye minde looked at him suddenly and nodded: "what you said is that you will give me their money directly at that time, and say I asked you to do so. Who of them will give it to them with me, remember?"

"Hehe, that's what I think. When you go back, let them buy something they want -"

After eating rice noodles, Zheng Jianguo endured the hot feeling and said, he saw Ye minde point his hand to his mouth and say, "eating too hot is bad for the esophageal tube. You told me that. Now how can you forget it yourself? Wait until it's cooler -- "