Chapter 373

"Hehe -"

Before Catherine's warning, there was an ambiguous snicker in the office. Zheng Jianguo looked at Catherine with an increasingly cold face and knew that he had to speak at this time: "is there anything ridiculous?"

“——”

Zheng Jianguo's voice was neither loud nor heavy, but as he spoke, the laughter in the office quickly disappeared. He leaned against his desk and said, "I know there is a saying that this thing only exists between gay men, but what I can tell you is that so far, I have contacted two women with this virus.

One of them has no health problems. I classify her as a virus carrier without disease. The other has symptoms similar to those of patients in our ward, such as enlarged lymph nodes, cysticercosis carinii pneumonia, skin rash, Kapo and tumor, and various malignant infections.

Of course, at present, only patient 3 has completed the pathological anatomy. Due to his gender, what we can confirm now can only be speculated based on blood and body fluid, that is, what Catherine calls intimate contact. This is the research progress, you know. "

"Boss, aren't you talking about our patient?"

As Zheng Jianguo's voice disappeared, he Dawei with a baby face raised his hand and said, Zheng Jianguo nodded and said, "this female patient is not our patient, because she has privacy concerns about coming to our hospital for treatment.

So everyone should abide by this rule. After leaving the hospital, outsiders are forbidden to talk about what you have studied, heard and seen in the hospital.

I think Catherine's previous suggestions to you are very necessary. Here I reiterate to you, because you will enjoy a rare holiday soon. You must do a good job in personal safety protection during the holiday, and there is still a bigger job to be completed after the holiday. "

"Vacation? Is there a holiday? "

"No, it's only been a few days -"

"It looks like a holiday -"

Zheng Jianguo's voice fell, and there was a whisper in the office. Marilyn had raised her hand and said, "boss, don't we study the shape and size of the virus?"

"It's not interesting. I've got a" surprise "ready for you. I'm afraid you'll complain."

Zheng Jianguo shook his head and sent the group of men away in a mysterious way. Soon after the office was empty, he watched Catherine and Esther stay, and then continued to say, "you two should see how to arrange shifts and rest. You should all rest next week."

"Oh, can you tell me what it is?"

Katherine looked at Esther and said that Zheng Jianguo didn't hide from them: "genetic testing. Next, you two should be responsible for the whole gene testing of unknown viruses and Helicobacter pylori. Let's see what they really look like."

"Genetic testing? Using Sanger and Coulson's chain termination method? "

Catherine and Esther were stunned by their Kung Fu, and two shocked cheeks appeared in front of Zheng Jianguo. He seemed very satisfied with their reaction and said: "there is also Maxim and Gilbert's chemical chain degradation method, but I intend to use Sanger method. Of course, in order to reduce the cost of PCR, I have entrusted someone to improve the PCR technology, A prototype must come out soon. "

"PCR technology improvement? How to improve? "

When Catherine blurted out, Zheng Jianguo shook his head with a mysterious smile: "it's still confidential now. Remember that even the information I told you both is confidential. Don't tell others, remember?"

"Remember."

Catherine and Esther Qiqi answered, and Zheng Jianguo said, "I'm going to New York this weekend. Do you two have anything for me to bring back?"

"Nothing to bring, thank you."

Shaking their heads, Katherine and Esther looked at each other, and both remembered that their homes were in New York. But if Zheng Jianguo was asked to bring something, it would certainly expose the fact that they had a good private relationship with him, so they all refused in order to avoid their families.

Although the difference between the two women fell into Zheng Jianguo's eyes, he did not have the idea of forcibly helping, but immersed in his sense of mission to promote the progress of gene testing technology.

PCR technology is not a new technology. Sanger invented chain termination method in 1977 to verify the reliability of PCR technology.

Before the Nobel Prize winner invented the chain termination method, he had done many polymerase chain reaction tests. He just didn't know whether it was the reason for the black light, so he didn't want to use computer and mechanical technology to improve the technology.

Until the human genome project proposed by Zheng Jianguo, his suggestions caused a lot of echo at that time, but after understanding the problems of cost, these echo disappeared.

Later, after managing several laboratories for half a year, Zheng Jianguo keenly realized that the loss of this post was the key to the test cost control, especially because there was no high-temperature polymerase before. If the temperature control was not well mastered in dozens of repetitions, there would be the risk of waste and failure.

PCR technology, to put it bluntly, is a simple DNA amplification method, which carries out denaturation annealing extension operation through the characteristics at different temperatures. The focus of this method is to master the water bath temperature. The polymerase will lose its activity when it is high, and the amplification cannot be completed when it is low.

The most important thing is the polymerase at this time, which is sold by gram. It is calculated by tens of dollars at a time. Under the operation of people, it is impossible to be foolproof, and it is easy to fail if the speed is not raised.

Therefore, the polymerase chain reaction operation before 1980 still belongs to the category of high cutting-edge technology, and even has a special position in the required laboratory to do this work, which limits the play of this technology in the biological field.

At this time, Zheng Jianguo had the idea of mechanical automation technology based on computer control in order to reduce the labor cost of PCR and improve the application field and success rate.

What Zheng Jianguo doesn't know is that Mullis, who invented the automatic PCR technology in his last life, completed the computer automatic synthesis program after the company arranged a similar job of synthesizing oligonucleotides.

Later, sometime in 1983, I thought this method could be used for DNA amplification, and after amplification, I won the Nobel Prize through the PCR instrument with automatic computer program.

Of course, at present, Zheng Jianguo knows nothing about winning the Nobel Prize with the PCR instrument. His idea is to reduce the detection cost after completing this thing, and then sell it to other companies for profit through commercialization. As for the Nobel Prize, he has no idea. The titles of the discoverers of Helicobacter Youmen and graphene have locked the Nobel Prize in advance.

This is why Zheng Jianguo will rest assured that Qian Yunjia will participate in this project. Otherwise, if he knows that this game will win a Nobel Prize, he will not agree to let outsiders share this award.

But even as a reborn, Zheng Jianguo couldn't keep his fat water from flowing into the fields of outsiders. He soon got off work, drove his Plymouth car and ran to the Biology Laboratory of MIT.

But he didn't want to see no one inside after Zheng Jianguo opened the door of the office. So when he came to the laboratory emitting disinfectant, he saw seven or eight pairs of eyes looking at it in an instant, and couldn't help but get a little confused: "what are you doing?"

"Success, founding of the people's Republic of China, come and see -"

Ye minde's ruddy face waved, but Zheng Jianguo was a little suspicious. In front of several people, "it's not Dr. Qian's high-temperature polymerase that has already passed the test?"

"This is the PCR instrument you want. You don't have to ask after setting the material."

When ye minde finished speaking in a loud voice, Zheng Jianguo had walked to the table surrounded by several people. He saw a black-and-white display connected to the next button, and there were dozens of grid placement holes. His face immediately changed: "can this be completed automatically?"

"Yes, there is a water bath. You only need to set the temperature and the time of temperature switching to control the number of times of multiplication, and then you can take it at the time. Of course, this is an engineering prototype. If you agree, I can design a beautiful appearance for it, but it requires an additional charge -"

With the sound of his voice, Zheng Jianguo found that in addition to the staff in the laboratory, there were two young people who were tall but unfamiliar. At this time, she found that he looked at them, but Celia spoke first: "boss, these two are my friends. They are graduate students majoring in electrical Engineering in the college. Stephen and Ryan -"

"Oh, thank you, Stephen, Ryan."

Zheng Jianguo seems to understand why Qian Yunjia completed the purification of polymerase yesterday, and has completed the automation design today, and also built the engineering prototype. The graduate students of electrical automation at MIT did it, which is not a big question: "I want to ask, what is the cost of this engineering prototype? If you were to put a shell on him. "

"About $300? The most expensive ones are the display screen and two CPUs. Do you know what CPUs are? Is it a logic chip that controls time and temperature? These three parts are more expensive, about $200. "

Stephen looked at Celia and Zheng Jianguo again. His face showed an inexplicable surprise. Suddenly, he said in a deep voice. Unexpectedly, Zheng Jianguo had found his wallet from his pocket. Celia next to him seemed to know what he was going to do and said, "they're here to help for free."

"No, there's no reason to let friends help for free, unless you really don't have money."

Zheng Jianguo smiled brightly at what Celia said. His fingers had already touched five pieces from his wallet. Franklin handed them out in a gentle voice: "I'm surprised that you can do it so quickly. I don't know if you can give me this design picture? The money bought your design at that time -- "

"This money is really not so much -"

Stephen looked at Qian in an instant and said hesitantly. Zheng Jianguo knew it was in his eyes only from his face. He immediately put the money in his hand and said with a smile, "let's make a deal."

"Well, this design is actually very simple."

Quickly put the money into his pocket, Stephen felt that he had said less before. He just remembered that he really wanted to help before. He looked at Celia next to him and finally said, "then we will send you the design and this shell as soon as possible -"

"Well, please."

Tucking his wallet into his pocket, Zheng Jianguo shook hands with the other party again. He was relieved. He looked back at Ye minde and said, "since it has been successful, Tao Ye will write the paper under the guidance of the teacher. If the paper is signed, you, Tao Ye and Qian Yunjia."

"That's not good. I said --"

When ye minde frowned, Zheng Jianguo smiled and looked at Qian Yunjia next to his eyes. Looking back, he said, "teacher, I entrusted you and Tao Ye to develop the PCR instrument in the name of Jianguo company. After the successful development, the technical ownership is owned by Jianguo company.

However, you have the right of authorship of the first author of the paper and are also the main designer and inventor of this project. Now with the participation of Qian Yunjia, especially with her joining, the development of PCR instrument has more than doubled.

Therefore, in terms of emotion and reason, we can't ignore her role in this project in the paper. If we can't ignore her role, then you and Tao Ye's role can't be regarded as not seeing it. Are you right, Qian Yunjia? "

"Yes, boss."

Qian Yunjia looked at Ye minde with a complicated look. As the discoverer of high-temperature polymerase, she has been acutely aware of the role of this PCR instrument, which is a revolution in the existing biology!

Even in the depths of her heart, Qian Yunjia regretted that she had promised so easily that she didn't even mention the conditions at that time. However, the employment agreement was signed, and it was too late to say anything at this time. Therefore, under the gaze of Zheng Jianguo, she opened her mouth and started ye minde's work: "Professor Ye, boss is right. Although I don't know how you used to distribute in China, But now in the United States, we have signed an agreement to do research expenses -- "

"Well, since you have determined the founding of the people's Republic of China, we should divide our contributions according to the facts. You put forward your ideas and ideas, and Tao Ye and I designed your ideas and ideas.

Otherwise, don't me and Tao Ye, just Tao Ye himself. She's doing your ideas and ideas. I just play a guiding role next to her. Besides, it's no use asking for these names when I retire at my age.

As long as you two still recognize me as a teacher in the future, I'm happier than I was on the lancet, and Qian Yunjia's contribution is the remaining one-third. The purified high-temperature polymerase simplifies the operation of the PCR instrument. How do you feel about this division? "

Seeing that Zheng Jianguo's cooperation was completed, ye minde put away his smile. In his heart, the first must be Zheng Jianguo, whose ideas and ideas were put forward by him. The second is that he and Tao Ye, as well as Qian Yunjia, who brought high temperature polymerase, are tied for the second place.

For himself and Tao Ye, ye minde knows that he is in this matter, just like Zheng Jianguo's discovery of the role of Helicobacter pylori, which can be regarded as a side point, and this is also his positioning to help Tao Ye grow up as soon as possible.

Then at this time, according to the contribution, ye minde felt that when Zheng Jianguo wrote his H. pylori paper at that time, he could give up signing in the first author in order to send Zheng Jianguo out. At this time, if he appeared with Tao Ye, or even with Zheng Jianguo, it would be against his original intention.

Moreover, ye minde believes that the role of this PCR instrument is not as important as that of Helicobacter pylori. At the beginning, all papers on Helicobacter pylori can be abandoned. For this unimportant invention and design, he still values the attitude of Zheng Jianguo and Tao Ye.

For Zheng Jianguo, ye minde has been regarded as the most proud disciple in his life. This does not mean how much money Zheng Jianguo has made and found, nor does it mean that he has donated millions to China, nor does it mean that he has invested hundreds of millions to build an airport!

What makes Ye minde value so much is that Zheng Jianguo didn't give up his goal before he became rich and famous! Not to mention Zheng Jianguo's current wealth, the millions and millions he donated are enough for ordinary people to live a carefree and even drunken life.

When there is no money and conditions, it is natural for people to endure hardships and struggle in order to improve their living conditions. However, after having money and improving their life, they still maintain their original intention of diligence and upward, which is commendable in Ye minde's view.

He has retired. Ye minde now brings these graduate students not so much to train another group of talents as to find some younger martial brothers and sisters for Zheng Jianguo. In this way, he will always have a helper on this road both at home and abroad.

Of course, the most important thing is that ye minde thinks that the first author is of little value. If he is famous, he is now a famous person in Qi City under the name of Zheng Jianguo's teacher. No matter how famous he is, it is nothing more than a compliment, but there is no benefit.

Secondly, ye minde also doesn't like the practice of competing for merit with Zheng Jianguo and Tao Ye, and tied for the first place with the younger generation?

Therefore, for these reasons, ye minde made a choice without much effort, and after making this choice, he felt that he was right to retreat: if this project is really as important as Helicobacter pylori, he taught Tao Ye again!

In that case, what are you unwilling to do as a teacher who teaches and dispels doubts?

For Qian Yunjia, ye minde believes that her contribution has played an important but not critical role in the invention of the PCR instrument. After all, he believes that he can find the high-temperature polymerase as long as he takes the trouble to find it, and Qian Yunjia's addition only accelerates the process.

Therefore, ye minde looked at Qian Yunjia specially after saying this: "if you really count, Dr. Qian's contribution is greater than mine, and in the future, as this project goes into business, her high-temperature polymerase is a hen laying golden eggs -"

"Well, teacher, you are humble again. Then do as the teacher says -"

Suddenly, it seemed that he was back to the scene of seeing Bruce for the first time. At that time, ye minde neatly pushed off the first author on the paper. Zheng Jianguo was a little impatient when he finished this sentence, so he turned to Qian Yunjia next to him and said, "Dr. Qian, if you give up the signature of the first author, But give you the right to use the technology of high-temperature polymerase. Are you willing? "

"No, you can't go back on the agreement. I'm afraid you'll file a lawsuit with me."

Qian Yunjia frowned with surprise on her melon seed face. She thought that when she signed the agreement, she gave up the ownership of all research results and only retained the signature right of technical research results. Moreover, based on her understanding of biology, she knew that the invention of the PCR instrument would definitely have a far-reaching impact on the discipline: "moreover, I am very optimistic about the commercial prospect of PCR instrument, boss. "

"Well, now that everyone has decided, sign as the teacher says."

Zheng Jianguo frowned instantly. At this time, he had the same understanding as Qian Yunjia, but ye minde insisted on giving up his signature. He felt that he should have said it in private, but now the old man has made a decision in front of so many people. It is obviously impossible to go back afterwards, So he threw the matter abroad: "it's too late for you to regret, Dr. Qian."

"I won't regret it. I feel that this PCR instrument should not simply help researchers multiply DNA -"

Qian Yunjia shook her head and said again, as if to strengthen her confidence and correct Zheng Jianguo's understanding of PCR. Her voice was sonorous and powerful: "its simplification can liberate a large number of researchers from mechanical repeated operations, so as to reduce a large number of human costs and use these manpower in other places, Moreover, adding high-temperature polymerase at one time also saves the waste of adding many times -- "

"I know that my laboratory at the Massachusetts General Hospital should have its place. Of course, it has to be Stephen. They will make some prototypes first. Celia, you need to sign a formal contract with them. I'll ask a lawyer to deal with patents -"

Looking at Qian Yunjia silently, Zheng Jianguo wanted to use the purified high-temperature polymerase technology in exchange for her giving up her identity as the first author. After all, he was not short of money, but he didn't expect that the eldest sister and normal researchers' pursuit of technology was higher than material pursuit, so he felt that this was also acceptable, Although he was vaguely aware that the technology had brought some innovation.

However, Zheng Jianguo still has a lot to do at the moment, and the reward for the research results is completed. Then the next thing is the material reward: "in view of the early completion of the PCR instrument project, I decided to give bonuses ranging from 10000 to 1000 dollars, Tao Ye and Qian Yunjia 10000 dollars, teachers and Yi Jinzhi, Wei Yongqiang and Celia 1000 dollars."

"Jianguo, this money is too much."

Zheng Jianguo's voice didn't fall, but Tao Ye opened his mouth and shook his head. He didn't want Ye minde to say, "Tao Ye, this has long been signed on the agreement."

"Yes, when the agreement is signed, we will follow the agreement -"

He took out the checkbook and signed the check. Zheng Jianguo gave Celia and Qian Yunjia the check. Finally, he gave Ye minde the check for $13000. With a smile on his face, he said, "teacher, you can ask them to choose something to buy in advance and send it back first. Of course, don't buy TV sets. The TV sets here are different from those in our family. If you want to buy them, I asked them to buy it from Hong Kong Island and send it back. "

"OK, Jianguo."

Silently put the check in the handkerchief and wrapped it up. Ye minde looked up at Qian Yunjia and Tao Ye, and then said, "then I'll take them to write a paper, or publish it as soon as possible."

"Then I won't bother you. Congratulations."

Zheng Jianguo looked at what he had to say, and then came to Ye minde and said, "teacher, do you think this achievement can be sent to science or nature?"

"Well, I feel almost nothing."

Ye minde looked at Qian Yunjia again. He wanted to say that he had never been in contact with these two top journals. In addition, in his heart, these two journals are the pinnacle that all researchers in the world dream of. Think about Zheng Jianguo, who has just climbed to the world by virtue of the integer Hall effect of an atomic graphite layer. He really has no bottom: "Dr. Qian, How do you feel? "

"Oh, Professor Ye, what did you say?"

Qian Yunjia, who was holding a check and secretly happy, obviously didn't hear what ye minde said. After approaching the two people, she listened to Zheng Jianguo again, but nodded affirmatively: "I feel almost like nature. The theory of PCR has long come out. The reason why Nobel Prize winner Sanger invented Sanger detection method is to detect the DNA fragments doubled by PCR, It's also a little innovative, but our PCR instrument is more inclined to engineering application, so I suggest submitting it to science first. "

"Then vote for science. If you can't, try nature again. Finally, if you can't, go to the cell."

Zheng Jianguo made a decision in an instant. He didn't expect Qian Yunjia to pay attention to Sanger. Of course, it may be that she saw relevant materials after joining the team, so she then said: "Qian Yunjia, you still pay attention to the research of high-temperature polymerase and just find Stephen and Ryan to do the design of large-scale production -"

"Oh, we're not selling technology? Do you want to do it yourself? "

Qian Yunjia was stunned again. Zheng Jianguo nodded: "PCR instrument and high-temperature polymerase will play the greatest value when they are put together, so I plan to set up a biotechnology company to further develop PCR instrument, so your high-temperature polymerase should continue, of course, if you like."

"Where are you going to put this company?"

Qian Yunjia asked what he cared about. Zheng Jianguo said without much thought: "the research laboratory is located on highway 128, which is not far away. Of course, the headquarters will be located in New York."

"Then I have no problem, but in terms of treatment -"

Qian Yunjia said with a smile you know. Zheng Jianguo smiled: "don't worry about this. Although I'm a businessman, my business philosophy is to make money. Your treatment is one-third higher than that of the head of the laboratory in Boston. Of course, this is one-third of the average."

"Thank you. You are a generous boss."

There was a brilliant smile on her face. Qian Yunjia just roughly calculated and knew that this was a condition for after tax treatment. Due to the dense gathering of scientific research institutions in Boston, the treatment of laboratory leaders here is generally much higher than that in other regions. Then add one third to the average value of these people, which still exceeded her psychological expectation: "thank you, boss!"

"If you really want to thank me, then work hard."

At the next moment, Zheng Jianguo showed his capitalist nature. After talking with Ye minde, he looked at Celia, who was silent next to him. He turned around and said, "teacher, although the development of the thermal circulator is completed, don't hurry back. You can also participate in the project on Celia's side. There is also a signed agreement between me and her."

"Oh -"

Turning his head to look at Celia, ye minde understood the purpose of Zheng Jianguo's continuing to invite them: "you mean the ointment?"

"Yes."

Zheng Jianguo didn't expect that the thermal circulator could complete the research and development so soon. Next, in addition to Celia's search for nitric oxide, that is, the application and development of nitroglycerin ointment.

Only in this way, it involves the choice of theoretical research and applied research. The purpose of the former is to focus on the search for intravascular nitric oxide, while the latter is to study the effect of nitroglycerin on cavernous vessels through experiments.

Ye minde suddenly thought of Tao Ye and Yi Jinzhi. He once heard Zheng Jianguo talk about nitroglycerin ointment. Although everyone is a doctor or researcher, he thought about the scene that he and Wei Yongqiang watched the two women go to experiment with animals, or the scene that the two women watched the two men do experiments with animals, It's really not a thing: "Yongqiang and I are still in charge here. Let Tao Ye and Yi Jinzhi learn from Celia."

"OK!"

Seeing ye minde make a decision, Zheng Jianguo found Celia and told him about it. The reason why he was embarrassed was that Tao Ye and Yi Jinzhi had different cultural influence since childhood. Chinese people had a conservative attitude towards x engraved into bone marrow. When facing this kind of experiment, they would inevitably have psychological discomfort. Unexpectedly, ye minde immediately understood his scruples.

Of course, Zheng Jianguo doesn't know that this is still caused by his traditional ideas. Otherwise, it's OK to straighten out her work attitude. Just as Celia didn't express too many opinions after hearing his arrangement: "don't say learning from me, we can learn from each other."

"Yes, you learn from each other and see if you can rent another laboratory next door. I plan to let the teacher and Wei Yongqiang be responsible for the experiment of nitroglycerin on animals -"

Zheng Jianguo said slowly, but Celia blinked her big eyes and said, "do you let them be responsible for the animal experiment?"

"Yes, it's mainly to study the effect of nitroglycerin on animal cavernous body. I don't think it's convenient for you girls."

Zheng Jianguo tried his best to keep the look on his face unchanged. He saw that Celia's eyes suddenly widened with a frown. She was a little surprised on her white oval face, and then her voice decreased a lot: "this, we can do --"

"Do it in front of other men?"

Subconsciously, Zheng Jianguo woke up and said something ambiguous. Sure enough, Celia looked stunned and her cheeks looked different. It seemed that after understanding his idea, she said, "listen to you. Anyway, you are the boss. Nitroglycerin can promote the relaxation of heart blood vessels and accelerate blood flow, Do you think it can also help relax and accelerate the flow of cavernous blood vessels? "

"Yes, I think so, so I want the teacher and Wei Yongqiang to do the experiment."

He nodded slowly. Zheng Jianguo took the idea to himself. He didn't want to see Celia's white cheeks. A pair of blue eyes looked at the zipper under his belt. His face suddenly seemed to understand something: "I know!"