Chapter 6

Cai Zixuan looked around and said, "the leader of our group is Wang Ruoxiang."

"In this case?" Gu Jun reluctantly frowned and thought it was something, "it's no surprise at all. It's not her that makes me wonder."

"Yes, that's Wang Ruoxiang." Cai Zixuan was filled with emotion.

Wang Ruoxiang is the monitor of their class. He has strong ability, beautiful person and good character. Such a goddess of learning hegemony, of course, pursues her from the teaching building to the Bureau building. Tu Haojun is one of them. In those years, she was called "magnificent", but she finally refused to finish with Wang Ruoxiang in public: "I'm sorry, we are not the same kind of people, I won't like you."

Well, a tall, handsome, rich, poor man with eight years' schooling should be such a man?

At that time, everyone said that thanks to Tu Haojun and the monitor, let them believe in love again.

"The monitor is still single now." Cai Zixuan obviously doesn't know how old-fashioned he looks now.

"Don't look at me like that." Gu Jun laughed at himself, "it's all over." Indeed, it was all over. Tu Haojun's pursuit of the monitor was somewhat like it, and more importantly, he wanted to catch up with everyone's goddess. In the final analysis, it had nothing to do with those profound things.

"Yes." Cai Zixuan did not continue to talk about the monitor. He looked at his watch and said, "ah, it's almost 8 o'clock. I have to go to the laboratory."

"Come on, I'm going to the lab building, too." Gu Jun said.

Cai Zixuan suddenly showed his understanding eyes. He just said that he didn't care about the monitor. He's going to join the party. It's really nice to be young.

"What?" Gu Junzhen was helpless. He wanted to explain and didn't know where to start. "Forget it..."

"Hao Jun, you don't have to say, you understand. I've been through the sea, but it's hard for water. I understand."

"You know a sponge."

The medical laboratory building is located in the northwest of the campus. It is a ten story white brick building with no special appearance. Even the front door is a dilapidated and dilapidated concrete floor. But inside the building there are all kinds of well-equipped laboratories for teachers and students to use in scientific research.

"This way." After entering the building, Cai Zixuan walked in front of him, introducing the situation to Gu Jun while walking.

The subject of their group's experiment is called "Research on the photodynamic treatment of tumors with several photosensitizers". It is a study by implanting tumor subcutaneously in mice, then photodynamic therapy with different photosensitizers, and then observing the changes of tumor.

This time, only 30000 yuan is allocated, which is much more for an undergraduate's scientific research experiment. However, an imported pure Photofrin photosensitizer can cost tens of thousands of yuan. In order to make more photosensitizers, Wang Ruoxiang made all kinds of careful calculations and saved the capital contribution. Then he set up five groups of mice, including the domestic imitated Photofrin group, the dotipofen group, the ala group, and the blank control group.

"The research plan and proposal report were all completed by the team leader." Cai Zixuan can't help but sigh, "it's really Wang Ruoxiang."

Although Professor Gu is a tutor, he only takes time to guide him occasionally. It's his business to write SCI articles with high scores. Therefore, the pioneer cup still depends on the students themselves. The team leader does the most work, but it is an opportunity that everyone can't get.

Because if the experiment produced a paper, Wang Ruoxiang was the first author, and Cai Zixuan could only be at the back.

"We are still in the first stage, which is to establish ascites tumor bearing mice model. We have been cultured for a week, but we can't see it today."

Cai Zixuan said a lot, and suddenly found Gu Jun didn't speak for a long time. He seemed to frown when he heard the word tumor

"Er, Haojun You know, the success rate of modeling is not 100 percent. "

Cai Zixuan scratched the bald spot in front of his head. He really didn't know what he had just said. Did Gu Jun understand.

"What can be 100% in the world?" Gu Jun lowers his head and covers his face. My friend, I haven't learned the dregs yet, OK.

"Indeed." Cai Zixuan was very touched by this sentence, and his face was filled with vicissitudes. "All the methods of action are like dreams and illusions."

Well, Gu Jun can't understand

During the conversation, the two took the elevator to the eighth floor, where their team used the laboratory.

Along the corridor, to the door of the laboratory, Cai Zixuan pushed open the door rate to go in first, only to see the members of the team arrived, he became the last.

"Zixuan, I'm waiting for you."

"Eh?"

At this time, people saw a person who came into the laboratory, and the only sound left was the mouse chirping.

Tu Haojun?

Didn't he quit school to travel around the world? Didn't he say he hung up? Not that he

"Hello, everyone." Gu Jun waved his hand to the crowd with a smile and looked at Wang Ruoxiang, "how are you, monitor."

Wang Ruoxiang stood at the edge of an experimental platform. She had dark short hair and a delicate melon seed face. Although she was plain, she was very beautiful. She was tall and wore a white coat. She was full of heroism. Wang Ruoxiang saw him, slightly frowned, "Gu Jun hello." It's a nice voice, too.Gu Jun nodded to answer her. After three months, the monitor was still so beautiful, but he was no longer.

"Hao Jun had to leave school before, but now he is back." Cai Zixuan explained to the public, and introduced Gu Jun: Xu Hai, a clinical classmate, was an acquaintance. Zhang Haoran, a basic medicine major, and he Yuhan, a pharmacy major, were all present.

Cai Zixuan asked: "monitor, Hao Jun wants to have a look with us. Is that ok?"

Wang Ruoxiang quietly pursed her mouth, then said: "stand aside and don't do anything."

Tu Haojun. She murmured in her heart that she had a superficial affection for him at the beginning of school a few years ago Nauseous. This kind of person is lazy and has all kinds of poisons. Rotten wood can't be carved, and mud can't help up the wall Mice are not so good! Maybe only by being pulled out of the slices for research, will it contribute to medicine.

"No way." Gu Jun stood aside obediently, but always felt that Wang Ruoxiang's eyes were not good Illusion.

On the experimental platform, there are a number of mouse cages, each with a group of white Kunming mice. This is the most commonly used experimental animal, very gentle and small, 1-1.5 months old, weight is only 18-22g, body length is less than 10cm, it can be used for experiment.

In addition to a cage of model mice, the rest are all blank mice, which Wang Ruoxiang just bought from the school's experimental animal center. Once the modeling is successful, they can come in handy.

Now that all the members of the team have arrived, they start to work immediately.

Crackle! Wang Ruoxiang put on rubber gloves and went to the cage of the model mice to catch the mice. These mice were labeled with the number of staining method, and she caught No. 1 mouse. Cai Zixuan and other people were in charge of assisting, recording, videotaping and so on.

"On the eighth day of intraperitoneal implantation of S180 mouse fibrosarcoma." Wang Ruoxiang looked attentive and serious. She gently pressed the abdomen of No. 1 mouse, which was obviously bulging up. Then the mouse gave out a painful chirp. She held it firmly in her hand.

"The ascites of mice No. 1 continue to grow. They are already frog bellies. They have good vitality and do not need to be executed for the time being," she said

Hearing what she said, the members of the team were all excited and rushed to see clearly, "is this the frog belly?" "Great."

"It's a frog's belly. It's really big." Cai Zixuan sighed.

Although they are all eight-year students, it is the first time for them to do such a real scientific research experiment.

"Oh." Gu Jun also came to have a look, or understand what they are doing.

The reason for producing ascites tumor bearing mice is to obtain a large number of high activity S180 tumor cells. We should know that the original cell line is very expensive, but one tube can only inject one mouse, and its activity is very weak, and their four treatment groups need to cultivate hundreds of mice.

So it's manufacturing first, then expanding. Now mice grow frog abdomen, can "collect ascites.".

"Gu Jun." Wang Ruoxiang suddenly turned his head and glanced at him, "can you go away? In the way. "

"Yes." Gu Jun shrugs his shoulders and walks away. Your aura is OK. It's a meter away from you.

Cai Zixuan and they did not see, Wang Ruoxiang continued to operate.

She inserted a 5ml syringe into the abdominal cavity of No. 1 mouse, successfully extracted a full tube of yellow clear ascites, and then injected it into an Eppendorf conical bottom centrifuge tube, which she then centrifuged. Once centrifuged, immediately you can see a large number of white sediment at the bottom of the centrifuge tube, that is tumor cells.

"Mouse No. 1 was successfully established." Wang Ruoxiang was relieved and happy, and said with a smile to the members: "ascites has a large number of S180 tumor cells!"

"Yes "Ha ha." They were young people, no one paid attention to the mouse's feelings. They cheered, and Cai Zixuan praised: "take the first step of success!"

The first step was successful, and then the cells were given to the treatment group to produce subcutaneous tumors, which were then treated with photodynamic therapy.

There are still many things to do. They continue to work, remove the supernatant from the centrifuge tube, re suspend the count, adjust the cell concentration

In order to maintain cell activity, complete the injection within an hour. So they were busy with one model mouse and then on to the next.

"Adjust the cell concentration to 1 × 10 ^ 7ml..."

"Yuhan, you are responsible for taking 0.2ml cells per needle."

"Chief, the anesthetic is ready!"

They all have high morale, and no one can care about Tu Haojun. They even forget to have him standing by and watching.

Gu Jun watched quietly, watching he Yuhan match cell suspension, Cai Zixuan injected pentobarbital sodium intraperitoneally to anesthetize healthy mice, Xu Hai and Zhang Haoran labeled and numbered the mice and depilated their buttocks. Finally, Wang Ruoxiang injected tumor cells subcutaneously into the right buttock of the mice

One, two, three, one after another.

All of a sudden, Gu Jun's head cracked and hurt. Was it a brain stem tumor attack

The pain suddenly intensified, and he couldn't help but cry out, "ah!" The scene in front of him became trance and flickered like the shadow of a nightmare.It seems that he can't breathe air. He seems to be in a dilapidated laboratory. Centrifuges, microscopes, sterilization pots and other instruments have fallen to the ground. Broken test tubes, messy documents, fuzzy photo frames, rotten animal bodies Dust turns all these into gray colors. There is no breath of life, only chaos and stillness.

The pain of the brain is more and more serious, but the scene is more and more clear, he can see some unspeakable devices.

And the twisted bodies on the ground It's impossible to identify the animal

He also saw that there was a line of blood characters on the wall inside, which was the mysterious language on the medicine box. However, compared with the standard of printing, every stroke of that line of blood characters had a kind of creepy strangeness.

In a trance, Gu Jun suddenly seems to be able to understand the meaning of the blood word.

He read it softly.

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