Chapter 8

Zhang Lin took out the container from the liquid nitrogen tank, then took out a cryopreservation tube from the container, and then put it back carefully, because there were still many cryopreservation tubes for other cells. After sealing the lid of the liquid nitrogen tank, Zhang Lin began a series of patient and meticulous operations.

First, he put the cryopreservation tube in a water bath pan, thawed it quickly with 37 ℃ hot water, then changed the centrifuge tube and centrifuged it for five minutes. Then he sat down beside the ultra clean working table, baked the tube mouth of the centrifuge tube to the alcohol lamp for disinfection, discarded the waste liquid, added fresh culture liquid with a pipette gun, repeatedly blown and mixed

Gu Jun has been watching carefully. If he has any questions, he asks elder martial brother Zhang for advice and learns a lot.

For example, the speed control of centrifuge, the suction and discharge control of pipette gun, etc., these resuscitation operations will affect the survival rate and proliferation rate of cells.

Finally, Zhang Lin injected the cell suspension into the culture dish, added appropriate amount of culture medium, and then put it into the cell incubator with 5% CO2 at 37 ℃.

"Wait, for at least two days." Zhang Lin took off his mask and pushed his glasses. "I suggest you keep it for a week, so that the tumor rate can be guaranteed."

"Try a batch of them for two days." Gu Jun shrugged and said that there is no way. There is not enough time. The system shows that he will get worse in 30 days. If it is true, it may even be difficult to get out of bed and walk. He added: "elder martial brother, would you like to go out for a meal in the evening? It's my treat

"No Zhang Lin waved his hand. "I'm going to give the mice medicine in the evening. It's my treat."

……

Gu Jun spent the next two days on campus. In addition to getting together with his friends, Gu Jun read books and prepared for the make-up exam. If he had to pass the grade if he could not die, it would be quite troublesome. The other was to go to the experimental building every day to take care of the cells and change the culture medium.

On the morning of the third day, Zhang Lin came to see the cells himself and said that the cells were barely growing. He helped to carry out subculture and prepare cell suspension.

Gu Jun went to the experimental animal center of the school. It was actually a large breeding base. It was hundreds of meters away from the school park. It was very lonely. It was probably because it smelled of various animals.

He bought five nude mice, each of which was more than 100 yuan, which was very expensive. Fortunately, he still had 20000 yuan in savings, but it could not last long.

When the money comes to use, Gu Jun really regrets that he donated the money so early People are still alive, money is gone.

Cough. Each of these nude mice is about the size of two or three fingers together, without hair. They are packed in a sterile box. Once they come into contact with the external environment with bacteria, they will easily be infected and die. Therefore, the process of breeding and doing experiments usually needs to be carried out in a barrier environment.

Dongda's barrier environment laboratory is located in the laboratory animal research center. This five story yellow brick building stands next to the experimental building.

Gu Jun and Zhang Lin met in the lobby on the first floor of the research center and ordered the right to use a small nude mouse room for one hour. This is a small room with only 21 square meters. It is divided into a front room and a back room. The front room has a test bed and instruments, and the back room is a feeding room.

Before they go in, they and all their belongings have to undergo strict ultraviolet sterilization. Two people with a special full body protective clothing, masks, gloves, foot cover and other complete equipment, this set foot in the nude mice room.

Soon, on the front room test bench, Gu Jun marked five nude mice with holes, and then injected tumor cells subcutaneously into their right buttocks.

Zhang Lin is in charge of assisting. These cells can form tumors when they hit people. Now seeing that Tu Haojun is so keen on nude mice, he is sure that this little brother is not trying to play murder.

Five nude mice were all injected, Gu Jun put them into the cage, and then to see if they can become tumors.

"Thank you very much, elder martial brother." Gu Jun gave a compliment to elder martial brother Zhang. Without elder martial brother's help, it would not be so smooth.

"The old Chinese medicine doctor you mentioned..." Zhang Lin is still full of Conan's vigilance, "is it really a liar?"

……

After a week, Gu Jun continued the previous rhythm of life, but went to the nude mice room every day to see the cage of nude mice. They are raised by the management of nude mice room unified care, each day accommodation costs 3 yuan.

This week, he has only completed three ordinary tasks, because those who need medical treatment can't do it. Although the hospital is nearby, he is not a doctor yet. His tasks are all related to medical practice. There are 5 boxes of pills in his hand, and his proficiency in Tairan's hands has reached the first level (30005000).

On the seventh day, Gu Jun went into the nude mice room with sterile equipment again and observed the nude mice one by one.

No one died. A soybean like tumor with a diameter of 0.5cm has grown on the right side of the buttock of No.1. There are also obvious protuberances in No.3 and No.4, with diameters of 0.3cm and 0.5cm respectively. However, No.2 and No.5 still show any signs of tumorigenesis.

At this point, he can confirm that five nude mice, three tumors.

Gu Jun set two tumor bearing nude mice as group A, one tumor bearing nude mice as group B, two non tumor bearing nude mice were divided into group C and Group D. in addition, he went to the animal center to buy 10 healthy nude mice, 5 in Group E and 5 in group F. Group A, group C and group E will receive the same dose of "strange drug" (named by him) every day, while group B, group D and group F will not be given any drugs, only for control purpose.In other words, tumor bearing nude mice, non tumorigenic nude mice and healthy nude mice have a group of drugs, and one group does not use drugs.

At this time, the experimental platform has been divided into six cages, nude mice are chirping very lively.

Gu Jun is fiddling with 25 capsules of foreign medicine. Since the system says that one box can prolong the life span of 5 days, a box of 5 capsules, it should be one capsule a day. This is the dose for adult humans, and the average weight of these nude mice is less than 20g. If they take one pill a day, they will die immediately.

He poured out all the powder in a capsule, weighed the dose, then converted the dose of nude mice with his own weight, made a solution, and then diluted it in proportion.

Soon, Gu Jun with a medicine filled stomach needle in one hand, and a fixed healthy nude mouse in the other hand, began the first intragastric administration.

"Haw haw." This nude mouse had a little struggle, but the poor thing was still pushed by him, making its contribution to medicine.

At the moment, Gu Jun's heart rate is a little fast, eyes blinking staring at the hands of nude mice.

He never tested the toxicity of foreign drugs until this point. If it dies suddenly, maybe the experiment is meaningless. And now, he stares for five minutes, then 10 minutes

Half an hour later, the nude mouse still did not appear any abnormal.

"It seems that the foreign medicine has no obvious acute toxicity." Gu Jun breathed a sigh of relief, "chronic toxicity should be observed again."

He then continued to fill the next nude mouse. Ten minutes later, he observed the other seven nude mice in the three drug groups. He observed them for another hour. All of them had no abnormal reactions. They could still call, drink water and eat feed.

"All rat friends, live on." Gu Jun murmured, pressed the painful head, his condition is obviously aggravating, "please."

……

The next day and the third day, Gu Jun continued to come to the nude mouse room every day.

The tumor size of group B nude mice without drug administration has increased from 0.3cm to 0.6cm, and the weight and mental state have not changed; the tumor size of two nude mice in group A did not change after two days, and maintained at 0.5cm.

Gu Jun knows what this may mean. The exotic medicine is really effective! These capsules can significantly inhibit tumor growth.

In addition, all the other drugs were given to nude mice as usual, which also meant that the foreign drugs did not seem to have any adverse reactions and did little damage to the body functions.

"Maybe it's really saved this time?" He thought that although the uneasiness still haunted his heart, a new hope was burning at the same time.

The fourth day, the fifth day In the twinkling of an eye, a week passed.

The tumor of group B nude mice has grown to 1.5cm. The huge tumor forms a sharp contrast with its thin body. It begins to lose weight and become poor in spirit. However, two nude mice in group a continued to be in stable condition, and all the treated nude mice were alive well, which was no different from that of the blank control group.

I have to say that the result of this experiment is very satisfactory.

The efficacy of foreign drugs is better than the top small molecule targeted drugs on the market.

However, Gu Jun also knows that the adverse reactions of foreign drugs can not be completely ruled out, which only proves that it has no side effects in a short period of time. After all, this is animal experiment, which is different from human clinical experiment.

However, the result of this experiment is worth his risk.

This afternoon, Gu Jun wrote his last note and went to the waiting area of the emergency department of the Affiliated Hospital of Dongda and sat down in a rare waiting bench. Surrounded by a sea of patients and their families, mostly crying children and comforting parents, nurses hurried up and down, nursing workers pushing the rescue bed

In a hustle and bustle, the emergency department's Radio continued to shout: "No. 182, Chen Ziyang. 183, Wang Yuxuan. No. 184, Huang Zixuan... "

Taking a breath, Gu Jun resolutely threw a capsule of exotic medicine into his mouth, drank a mouthful of mineral water and swallowed it.

"Come on." He looked calmly at the emergency sign on the wall over there. "If there's any toxic reaction, all come."

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