Chapter 460

Yang Rui stayed in the sanatorium and put up the equipment with Liu Shan.

There are many ways to isolate strains. In 1879, the French isolated high-temperature strains from the Seine River. After more than 100 years of development, there are many ways to treat samples. The difference of times is more about simplifying the way and improving the yield.

Of course, the cost also increases.

Yang Rui first prepared centrifuges, which are no longer commonly used in biological laboratories, for centrifuging water and mud samples.

Under the action of 10000 centrifuges per minute, the impurities and supernatant of enriched bacteria will be directly separated from the water sample.

The isolated bacteria are of high quality, and the supernatant will be eluted through the filter membrane.

They can be screened by culture medium.

The principle of mud sample is similar, but it needs to use spring water to suspend first, then centrifuge at low speed to remove large particles, and then treat and enrich bacteria in the way of water sample.

The whole process is very simple in theory, but the equipment is not simple.

Centrifuges, which can rotate 10000 times per minute, are not cheap. They have smaller capacity. They can rotate more than ten test tubes in ten minutes, and it will take a long time to process 100 kg of samples.

The culture stage is also very expensive. Agar, glucose, peptone and so on used in the culture medium are much more expensive than the same weight of food.

In addition, the cultivation conditions of 70 degrees or even more than 80 degrees also put great pressure on the cultivation cost.

Kanghong helped Yang Rui to transport equipment and materials. When he took them apart, he found that only a little bit of competition was extinguished.

He didn't want to compete at all. It's not a new technology to isolate strains. What's meaningful is what strains can be isolated.

If a powerful strain is isolated, it will naturally be able to invent the paper of niubai, but if only a common strain is isolated, it will be difficult to be published in the entry-level journals of SCI.

The results are like buying lottery tickets, but the cost of Yang Rui's investment is so high. When Kang Hong looks at it, he feels that it lacks competitive value.

"Spending so much money to isolate thermophiles? Is it worth it? " Kang Hong thought of it and asked.

"The instruments are not bad. When we increase our manpower, they will still work." Yang Rui answered as he inserted the cable.

Kang Hong picked up a large box of filter membrane, put it in the corner, and said: "consumables, these are all ultrafiltration membranes. They are all imported, so much?"

Yang Rui "en" a, way: "I originally planned to deal with a batch, and then take back a batch, these costs are not saved."

"It's too expensive. We can't afford it." Kang Hong tut tut twice.

"If you're lucky, there's more left." Yang Rui said casually, and Liu Shan assembled the incubator.

There are many kinds of filter membranes, which are familiar to ordinary people. It should be the filter element in the household water purifier, which is also a kind of filter membrane. In the initial stage, these civilian products were all from the laboratory. In the 1980s, they were naturally valuable.

In fact, laboratories have higher requirements for materials and are not cheap at any time.

Yang Rui is very willing to spend money in order to isolate the thermophilic bacteria he wants as soon as possible. Kanghong has no reason to make such a large investment. Naturally, he can't understand it. He can only attribute it to the fact that people at Jingcheng University have too much money.

Yang Rui had to do the same.

It is a relatively common research work to isolate strains, but it is not necessary to isolate the desired strains.

In history, most of the antibiotics came from the isolation of soil strains. In the past 20 to 30 years, biologists have almost destroyed the soil all over the world. The most successful one is the tuberculosis terminator Waxman. He invented a systematic isolation method, and then got streptomycin, and then won the Nobel Prize.

Waxman organized as many as 50 research dogs for this purpose, spent several years, screened out more than 10000 strains, and got the one he wanted

Yang Rui can't afford to keep 50 experimental dogs and doesn't have a few years to waste. Fortunately, he knows which hot spring it is. As long as there are enough separated dogs, he can always find the breed he wants.

It took more than half a day to install a simple laboratory.

Yang Rui grabs a handful of soil from the ground, mixes some liquid, loads it into a centrifuge, and tests it.

Ten thousand revolution centrifuge, the noise is not small, but the sound of Kang Hong's heart is in full bloom.

"We also have one in our hospital, which is locked up with a wooden cabinet." Kang Hong stares at the machine in the transparent glass, and suddenly says.Yang Rui understood: "public laboratories in schools are like this."

"So is Peking University?" Now the information is backward. The distance from Yunnan to Beijing is even farther than that from Beijing to Los Angeles. It can catch up with Fuzhou to Taipei.

Yang Rui recalled for a moment, and said: "almost. The administrator of the public laboratory only needs the instrument to be in good condition. He doesn't care whether it's convenient for you to use it or not."

"That is to say, we have to apply for a good machine at least two months in advance. If we can't get it right, we still have to treat it. After that, people say that we have to write out false invoices and false invoices for these grandchildren." Any researcher, when it comes to instruments, is full of fire.

When Yang Rui was a graduate student, he was also afraid of using the instruments of public laboratories. Of course, what he was most afraid of was borrowing the instruments of other laboratories. It was not easy to look at others' eyes, and it was even more difficult to rub the instruments under others' eyes.

But those who have been in the laboratory for half a year have a deep understanding of the pain. Half of the reason Yang Rui tried to make money and build his own laboratory independently is to avoid the recurrence of the dilemma.

If it's just to meet his personal expenses, where can he use the trouble to buy a monkey ticket of 2000 yuan? After almost throwing it away and changing his house, he can be a 21st century billionaire at will.

However, it's just a billionaire who doesn't decide his own research direction and make his own experiments to be interesting.

If there is no huge pressure and personnel struggle, as the person in charge of an independent laboratory, it is quite a sense of achievement to do what he wants to do and really do it.

Kang Hong didn't have Yang Rui's experience, and he didn't know how to get rid of the current restrictions. He just asked enviously, "where are the instruments you took out? Isn't it provided by Peking University? "

"It's funded by Hong Kong companies."

"Hong Kong people are really rich." Kang Hong asked, "where did you meet them?"

"I wrote my paper and they were interested... Well, the centrifuge stopped. It looks good." Yang Rui left and got busy.

Liu Shan smiles at Kang Hong and starts to attack Yang Rui.

Yang Rui slowly serious up.

It's not worth money to isolate ordinary strains, but it's a good paper to isolate extreme thermophilic bacteria and then heat-resistant polymerase.

Influence factor 4. 0's "JMC" probably in front of the goal to lower a little bit, it must be no problem.

Theoretically, it makes sense to spend 600000 yuan on such a paper.

Although it is reasonable to say that the cost performance is relatively low, considering that it is only a necessary step of the paper, it is not at a loss.

Kang Hong saw that they were busy, so he had to leave unhappily.

In the afternoon, Yang Rui and Liu Shan finished their preparations only when the weather cooled down.

"I haven't come back yet. Maybe I'm camping out. What shall we have for dinner? " Yang Rui looked at his watch and found that camping was planned. The hot spring was in an undeveloped state. He only did a simple geological survey, and it was likely that he would not be able to go back and forth in one day.

Liu Shan tilted her head to think for a while, raised her hand and said, "eat instant noodles."

"Instant noodles? Well, it goes well with hot spring eggs. " Yang Rui cleaned up and went to the canteen to buy eggs and instant noodles.

At this time, instant noodles are still fresh goods that veteran cadres want to taste.

"Where to eat?" Yang Rui asked and said, "I'll find a waiter and ask him to iron our eggs."

"Together." Liu Shan carried a small bag, blinked her eyes and said, "we can make our own hot spring eggs. We can soak in hot spring, eat instant noodles and eat eggs at the same time."

Yang Rui surprised way: "men and women separate bath."

"That little yard is empty." Liu Shan pointed to the courtyard at the foot of the mountain not far away.

At the gate of the courtyard is the sign of "senior hot spring room", which is a small courtyard built around the hot spring pool. The introduced hot spring is steaming and belongs to the tenant alone. It can be soaked at night and day without using multi person dressing room.

Yang Rui said: "people are given by cadres at or above the department level. I can't use Li Zhangzhen's identity certificate."

Yang Rui has obviously tried. The staff of sanatorium insisted on their own opinions, so Yang Rui didn't insist.

After the full development of tourism resources, there is nothing rare about such hot springs. It is nothing more than a pool of hot spring water, more pipes for water supply and drainage, which can charge hundreds of yuan more for the room, stimulating countless hot spring resorts to participate in it.

However, in terms of the current state of resource development, hot springs are not rare. On the contrary, the construction cost has become a bottleneck. In addition, the sanatoriums that develop hot springs are all units with full financial allocation, and there is no impulse to make profits. After the construction of one sanatorium is completed, there is almost no new investment except for repair.An independent courtyard with built-in hot spring is a fresh experience in Tengchong. In the case of a small number, naturally we should be careful to keep it, so as to avoid the sudden arrival of senior leaders.

Liu Shan tightened the small bag in front of her chest and said, "there's no one inside. Let's go inside secretly."

Then, Liu Shan took the lead, bypassed the light at the intersection and picked it up from the low wall behind.

The sanatorium that poor people built for old cadres did not consider that there were residents who could climb up the wall.

Yang Rui looked at it for a few seconds, gritted his teeth and said, "what are we afraid of?" then he turned in.

Turning into the courtyard, Yang Rui was stunned.

In the bright moonlight, Liu Shan was stretching her waist and taking off her coat.

In the dark, what Yang Rui sees is a beautiful curve, and what he hears in his ears is a small sound.

……