Chapter 1456

It's hard to enucleate cells.

50 It's very difficult in the 1980s, and it's also very difficult in the 1980s. After the 21st century, it's still very difficult.

It can be said that any person, whether bachelor's degree, master's degree or doctor's degree, as long as they can master the method of cell enucleation, can easily find a job with a monthly salary of 3000 yuan in any full-time university!

For example, if Comrade Wang Liangcai goes abroad now, he can easily find a teaching position with an annual salary of 40000 yuan.

If you make an analogy, the work of removing and injecting nuclei into cells is like doing heart surgery in a hospital. Is there any operation more difficult than heart surgery? Of course.

However, in terms of universality and popularity, heart surgery is more eye-catching.

Wang Liangcai is the chief cardiologist in experimental biology.

His success rate is not high, but, under the microscope, a little bit of fiddling with the location of cells, and then carefully suck the nucleus of the people, few people in the country.

Wang Liangcai worked in the laboratory for several days, and everyone expressed their admiration.

The laboratory, like the factory, is the best place to show the ability.

Because we all make things in kind, and the workplace is in the same place. You don't need to show off whether the things you make are good or not. You can see them with open eyes.

In fact, in order to show their vision, people who can see have to help with publicity.

Therefore, a good strong man in the laboratory is just like a good level 8 worker and level 7 worker in the factory. He can be easily found and praised.

In addition, Wang Liangcai is older and modest. Not long ago, even research dogs are used to asking him for advice.

"Professor Wang, can you take a look at this for me?"

"Professor Wang, there is always a white spot in the image that I scan with electron. Can you help me to have a look?"

"Professor Wang, I have an idea recently. It's like this..."

"Professor Wang" sounds to the ear, but Gu Qiang is very uncomfortable.

Once upon a time, these questions were all asked by him. At least, some of them were asked by him.

Although I thought it was troublesome at that time, no matter how annoying it was, it was my own dog. Now what's the matter with adding other people's thighs?

"Professor, I want to apply for a little more time." Gu Qiang can't help but bypass the big work area and ask Yang Rui for the policy.

Yang Rui always looked down at the expression of the paper, only raised his eyelids and asked: "your machine time is not enough? What kind? "

"Inverted phase contrast microscope, in addition, pulse meter, I also want more machine time." Gu Qiang answered quickly.

"Oh. How much. " Yang Rui points the table with his hand and puts down the paper.

It's normal to run out of time. It is impossible for any laboratory to supply instruments in unlimited quantity, and even for public laboratories, they seldom buy expensive instruments repeatedly.

In this case, sharing machines is the best solution.

Of course, if Yang Rui was doing the project himself, he would certainly have to use the opportunity. However, Gu Qiang could not have such good conditions to do the experiment.

Like other researchers, he has to apply for the machine, and then carry out the experiment within the specified time. If it can't be completed by the due date, and it can't be occupied by the overdue date, we have to discuss with the later researchers.

Or, as it is now, apply for more machine hours.

Gu Qiang glanced at Wang Liangcai and said, "four hours of microscope, two hours of pulser."

There was an inverted phase contrast microscope in the laboratory. Yang Rui didn't need to look at it. He said, "four hours is gone. If you work in the daytime, you can get two hours at most."

"That's not enough." Of course, Gu Qiang knows that there is not such a long time. At present, inverted phase contrast microscope is a high-end product. If you use it to brush papers, you can get an SCI in half a month, even though it is a waste.

However, in the genetic engineering laboratory, the utilization rate of inverted phase contrast microscope is already high. In addition, Wang Liangcai and others, who are active in their work, want to spare four hours, but basically have no solution.

Yang Rui shrugged his shoulders and said, "you can learn from Wang Liangcai and stay late at night or come early."

No matter how expensive the microscope is, it can't be compared with supercomputers and the like. In addition, the genetic engineering laboratory has not expanded to the level of departments and colleges, so there is no machine hour in the early morning. Wang Liangcai's early arrival is tantamount to seizing the extra opportunity.Gu Qiang was determined to compete with Wang Liangcai. Without hesitation, he shook his head and said, "I want to do a coherent experiment to connect the time of the day. You can get me a few hours, and I will take a week."

"Have you got an idea for the experiment?" Yang Rui looks at Gu Qiang in surprise.

Gu Qiang hardened his head and said, "if you have it, it's bad."

"Is four more hours enough?" Yang Rui has some confidence in Gu Qiang's experimental ability.

Gu Qiang continued to hold his head and say yes.

Yang Rui hesitated for a moment and said, "if you do this, you will be occupying other people's opportunity. Wait for me to ask."

Gu Qiang can't help but feel helpless. We have to discuss with others and, most importantly, with Wang Liangcai. This is not what he wants.

Yang Rui did not ask Wang Liangcai to come over and ask, but went directly to Wang Liangcai's test bench and watched his operation.

Wang Liangcai is doing the operation of nuclear display.

This is the first step of enucleation. You have to make the nucleus of the cell appear before you can pump it out.

If the enucleation experiment is compared to a happy playground, enucleation is the step of queuing up for tickets. It's boring. It's just that you keep fiddling with the cells with probes, and then you can fix them correctly with a fixed needle, and then you can do the fun part.

Sadly, the denuclearization experiment is not a happy playground. It's boring. Imagine how boring it is to show the nucleus.

However, Wang Liangcai is meticulous, and Yang Rui is meticulous.

With him, no one else will come.

Two people, just like this, did and watched for nearly an hour.

Yang Rui from the front to the back after watching the nuclear part, can not help but some emotion.

In another 20 or 30 years, there will be no fundamental change in the steps of denuclearization. At that time, how many people can do as well as Wang Liangcai?

The Biology Department of each school basically enrolls one or two classes of genetics students every year. With the accumulation year by year, can each school have at least one researcher who can do the cell enucleation experiment in 30 years?

Of course, that's impossible.

Just like most hospitals, there are no cardiologists who can handle it.

They may have one or two good doctors who can handle it, so as to set up one or two departments that can handle it, just like most schools have one or two major that can handle it.

But that's it.

As for Wang Liangcai's operation, it has far exceeded his ability.

Depending on the level of experimental biology, how powerful are the people who are selected by academicians? If you bind a white faced sheep and tickle him, he can make the sheep laugh all his life.

"All standard operations." This is the reason for Yang Rui's feeling.

It seems that everyone knows the standard operation, but when we ask, there are several people who can really do it all.

Just like the players on the basketball court, can professional players make standard operation?

If that's the case, are the varieties that hit less than 50% of the free throws grown on trees?

Compared with Gu Qiang, who relies on talent, standard operation needs more resources and more effort to practice step by step.

Sometimes, you can make good experimental results without using standard techniques, and you can change all your operating habits. That's how hard you have to be to yourself.

But Wang Liangcai didn't notice Yang Rui's praise. He said with a smile, "it's just like doing too much. We used to go to the laboratory to peel eggs when we had nothing to do in school. If the test-bed is not enough, we should make preparations for the experiment, and those who come and go are familiar with it. "

"I see your experimental record, the success rate is 30% Just like there is a notebook beside the hospital bed, there is also a notebook beside the test bench to record simple data.

Of course, it's high-end laboratories like genetic engineering laboratories that can record such details. Ordinary laboratories are like hospitals in cities and counties. It's good to have a dirty notebook.

Wang Liangcai was neither proud nor discouraged of the 30% success rate. He said calmly, "yes, I want to achieve a success rate of about 50%, and then continue to do the next step."

It is said that there are three major steps in nuclear display, nuclear removal and nuclear injection, which should be completed in one go.

According to the previous experience, the success rate of these three steps is not bad.However, the success rate is calculated by product. If Wang Liangcai's success rate of each step is 30%, only 2.7% will be left after three steps, which can reach the pass line.

Considering that the more difficulties we have in the future, those who can strive for a high success rate in the front still need to strive for more.

Yang Rui nodded and said, "well done. I heard that you often come to the laboratory in the early morning recently? "

"Yes..." Wang Liangcai's answer was not very firm.

It's not necessarily a good thing to come early. The discipline of the laboratory is very strange. As a seconded staff member, he is somewhat uncertain.

Besides, will those who go to the gym at four o'clock in the morning be praised? What if it affects the relationship between the coach and the manager?

"I'd like to adjust the time. If you want to come to the lab earlier, how about I leave the morning for you?" Yang Rui's answer was somewhat beyond Wang Liangcai's expectation.

"May I have it?"

"Well, just press your previous machine, or do you want more?"

"Can I have more?"

"One o'clock at noon at the most, will you?"

"Yes, yes." Wang Liangcai was overjoyed.

Morning is undoubtedly the best time for the experiment. If it's quiet, all the instruments are in the best condition, because at the end of an experiment, all the instruments that can return to zero have to return to zero, and there is less time to adjust the next day, which is both time-saving and comfortable.

A few days ago, Wang Liangcai came to the laboratory in the early morning. At eight or nine o'clock in the morning, he had to free up the inverted phase contrast microscope and other organic time distribution microscopes. If an experiment was not completed at the moment, he had to say good things to the researchers who came in the morning.

Now that the timing is connected, it's much more convenient for him.

Gu Qiang is completely helpless to listen to it. He knows that Yang Rui readjusts the time in order to free up the whole machine time for him, but... Why doesn't Mao give me the morning time?

"It seems that it's time to prove yourself with strength." Gu Qiang clenched his fist in silence.