Chapter 155

After the greetings, Zheng Lina, half joking and half serious, proposed to work here in Zhou Wen.

Zhou Wen had a little accident.

He was about to recruit another employee, but he didn't expect to send it to his door.

Tang Xin was supposed to have an assistant. In fact, it's almost enough. But recently, he's so busy that he doesn't have time to extract high concentration DNA.

So he decided to train a successor to help him make high-density DNA.

Tang Xin is naturally the best candidate. He has a deep understanding of his roots. In addition, he has a calm personality, which is very suitable for him.

Zhou Wen happily agreed to Zheng Lina's request to stay.

"Specific work content, let tangxin teach you later."

Naturally, Zheng Lina was overjoyed and nodded, "mm-hmm, I know ~ thank you."

Zhou Wen then told Tang Xin, "today I'll teach you how to extract DNA."

"Ah I'm afraid I can't learn. "

Tang Xin was surprised. Although she had dissected mice in University, she mainly did pharmacological experiments and did not extract DNA.

"Oh, it's very simple. It's not as complicated as you think."

Zhou Wen started teaching on the spot immediately.

A white mouse was taken from the cage and came to the console and said, "first of all, kill the mice. This is different from your pharmacological test. We mainly take the bone marrow of the thigh bone of the mice..."

How to extract more bone marrow from mice by Professor Tang Xin of Zhou Wen.

His lectures are very slow and detailed. These experiences are obtained from the theory and practice after he obtained the system, which is more simple and easy to understand than any other professor in the world.

Tang Xin can basically understand once.

Of course, DNA extraction does not rely on theoretical experience, without it, only by hand.

So after Tang Xin understood the principle, Zhou Wen directly gave up the operating platform, let her start in person, and he corrected the technique on the spot beside him.

Including the centrifugation time, the method of extracting supernatant, the frequency of blowing, the amount of fixed liquid added, etc.

Even Qin Haitao did not get this kind of teaching treatment

In the following period of time, Zhou Wen basically spent in the Institute of medicine.

At the same time of teaching Tang Xin's DNA extraction method, he also did the pharmaceutical task.

In addition, he pumped the air conditioner to prepare some of the best proportion of low permeability liquid.

And it is the key to ensure that the highest concentration of DNA can be extracted every time, otherwise only a little higher than the general DNA concentration can be extracted.

A week later, Tang Xin has been able to extract DNA independently. Although the concentration is not high.

But it's also a great thing.

When the mouse DNA was observed in the electron microscope, Tang Xin was very happy.

"Wow, great..."

……

……

Saturday, December 29, the day before New Year's day.

The prion research institute of Jiangzhou first people's hospital was officially established.

This is also the second prion research institute in China, and its scientific research strength is recognized as the first in China.

The first director of the Institute was Li Wenshuo.

Li Wenshuo, whose daughter-in-law has become a mother-in-law for many years, could not help but get wet at the opening ceremony.

He has been studying prions since his second year of study. Now it has been eight years and nothing has been achieved.

At the worst, I had to set up a stall with several colleagues because I couldn't apply for research funds.

Now think of it, but also a bitter tears.

It is for this reason that Li Wenshuo is particularly grateful to Zhou Wen. At the darkest moment of his life, he pulled him and arranged a chair for him on the rocket of heaven, which made him become a world-famous prion expert from a nameless little figure in the domestic academic circles!

After taking a group photo with some leaders and scholars who came to attend the unveiling ceremony, Li Wenshuo invited several peer scholars from home and abroad to go to the office to talk in detail.

As soon as they entered the office, they found that there was a big picture of the people on the wall.

"Well, this man is so familiar."

Li Wenshuo looked at the picture of Zhou Wen on the wall and said with a smile: "yes, he is the inventor of psc1, Zhou Wen, my idol."

Li Wenshuo is not only grateful to Zhou Wen now, but also worships this young man who is nearly 10 years younger than him. That's why he got a picture of Zhou Wen hanging in his office. It was like chasing stars.

Professor Qin Ziyu, deputy director of China prion research institute, regretfully said: "he clearly has outstanding talent in prion. I don't know why he doesn't continue to conduct in-depth research."

Li Wenshuo said with a smile: "I have asked him about this question.

He said that there are too many diseases in the world, and all kinds of viruses and bacteria are threatening human life and health, which often make him feel powerless.That's why he gave up studying prions and engaged in more advanced genetic research, hoping to crack the human genetic code and cure more diseases one day. "

Qin Ziyu sighed: "although I don't approve of his doing so, no matter what, his courage is commendable."

Li Wenshuo nodded, followed by a smile: "this morning, the United States MIT..."

Institute of cryptococcosis, 2333 Jiangzhou science and technology park.

The site renovation, equipment and personnel are all in place.

Fifteen newly recruited doctors in cell engineering also have a preliminary understanding of the project to be studied.

And the result of understanding is that everyone is confused.

Based on their level, it is impossible to clone antigen serum cells.

let alone that the content of antigenic B cells in serum is as low as a terrible number. Even if B cells are isolated, the requirements for sensitivity and specificity of sequence amplification and determination are very high.

Even if the sequence is amplified and the antibody is expressed, the final effect of the antibody remains to be discussed.

If they really have this level, they will not be able to find a job. They would have gone to Europe and America to earn dollar.

At lunch, Xiao Yang, the leader of the research group, went to Qin Haitao and asked, "do you know what Zhou Suo is planning? At present, the work can not be carried out at all, or there is no direction at all. "

Qin Haitao said with a smile: "be at ease. Don't be impatient. First, you should have a good understanding of Cryptococcus and the composition of antibacterial serum. As for how to unfold it, we will explain it in detail when we come back from the next week."

Xiao Yang said helplessly, "OK..."

Jinhu Industrial Park, No. 404, Huanyu Biotechnology Research Institute.

Unlike Xiao Yang, Wang Wei and his doctoral students have spent a very substantial period of time.

According to the requirements of the company, they have made a detailed understanding of domestic and foreign mainstream biotechnology, such as molecular biology, transgenic science, bacterial community, natural products and plant synthetic biology, clinical biological processing, etc.

There are 91 people, three people in a small group and 30 people in a large group. They learn from each other, communicate with each other and compete with each other. A good and orderly academic ecological environment has initially been built.

Such an ecological academic environment is the dream of all biological life in China.

However, the academic environment is very demanding.

In the past week, it has spent 210000 yuan, which is about 3000 yuan per person on average.

We should know that this is only the most basic guarantee for scientific research. If we go back to the laboratory after the opening of the laboratory, the cost will be increased by at least five times.

One million a week.

Four million a month.

Don't mention anything in a year. Scientific research alone costs at least 50 million yuan.

This is also the reason why Zhou Wen hesitated before.

The task seems simple, but in fact, the real difficulty lies in the operation and development behind.

If we can't develop the products as soon as possible, the Institute will close down soon.

At two o'clock in the afternoon, in the office of Hengfeng building, Chen Zhiyuan looked at the thick sheet of bills on his desk, with a painful expression on his face.

This period of time, such as running water, office space, office supplies, equipment and equipment, activity funds and so on, open your eyes every day is money.

He knew for the first time that the boss was under such pressure.

The first 50 million yuan has been paid in, but more than 3 million yuan has been spent.

This is "small money".

The real big head is in equipment procurement, and the bill on the table is the equipment list.

36 million! Two million advances have been paid.

For 10 sets of Hitachi hi tech s-3700n, Hitachi hi-tech s-4800, Hitachi hi-tech su-70, the total price of different types of electron microscopes exceeds 20 million RMB.

So terrible!

After checking the bill carefully, Chen Zhiyuan went to the financial office and asked them to make the final payment to the supplier.

I just came out of the finance room when the phone rang in my pocket.

Take out a look, it is a strange local number.

After connecting Chen Zhiyuan, he said, "Hello, this is Chen Zhiyuan. Who are you?"

"Hello, Mr. Chen. This is Meng Xiang, general manager of Jiangzhou Tianhai Intellectual Property Agency Co., Ltd. Mr. Zhou's patent application has been passed. He asked you to come and hand over some procedures."

Chen Zhiyuan said strangely, "what kind of patent?"

Meng Xiang: "the technological process of sildenafil granules."

"What, sildenafil?" Chen Zhiyuan exclaimed at the speech.

Meng Xiang: "yes! If you have time now, you may as well come to the company. "

Chen Zhiyuan even said, "OK, I'll go there now. Where is the address..."Hang up the phone, Chen Zhiyuan almost did not jump up.

He has eaten sildenafil granules, and the effect is amazing. If it is produced later, hehe

And Zhou Wen said that this medicine will be given to the worldwide agent.

Thinking of this, Chen Zhiyuan immediately left for Tianhai company.

When he arrived at the company, Zhou Wen followed him.

Then Meng Xiang's words, but let the two excited heart as if were thrown a basin of cold water, instantly cold down.

According to the FDA, the new sildenafil granules are already in the category of new drugs. If you want to produce and sell them, you need to go through the clinical trial process again.

Zhou Wen's dream of collecting patent fees was shattered.

According to the normal clinical trial process, it will take at least three years from human trial to marketing.

Chen Zhiyuan was speechless: "it takes so long?"

Meng Xiang nodded: "yes! The normal process is like this. "

Zhou Wen asked, "what about the abnormal process?"

Meng Xiang said with a smile: "there is another way to go through the green channel of rapid evaluation. If it works, the clinical trial can be completed in about 12 months.

After all, sildenafil is also a drug that has been tested in the market for many years. On the basis of unchanged raw materials and formula, it only improves the production process, so there should be room for operation.

You can talk to the cro company about this. They should have a way. "

Hearing Meng Xiang's words, Zhou Wen's eyes suddenly brightened, "yes, how can I forget them."

The so-called cro is called contract research organization, and its Chinese name is contract research organization.

It originated in the United States in the early 1980s.

It is a commercial organization that provides specialized services for pharmaceutical enterprises, medical institutions and small and medium-sized medical device R & D enterprises in the form of contract.

There are three types of industrial chain.

From the discovery of 0-pcc (preclinical candidate compound).

From pcc-ind (new drug clinical application) validation process.

Indnda (new drug marketing application) in human clinical trial stage.

In short, the goal of cro is to improve the efficiency of drug development.

You can think of all pharmaceutical companies as e-commerce stores, and cro is the e-commerce platform.

When it comes to cro, we have to mention the domestic "Yaoming Kant" and "Tago medicine".

For example, Yao Ming Kant is "Ali of cro world". Their slogan is: let the world have no hard medicine and disease

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