Chapter 356 - Tests For Animal Insulin

In the laboratory of the imperial palace, Einar was analyzing a reddish-yellow liquid. It was what he had obtained from the pancreas of a pig. He very carefully tapped the syringe.

This caused the blood to separate from the yellow liquid, later it began to release the red liquid, leaving only the yellow liquid.

"This liquid should be insulin, but it has to be diluted with alcohol to make it work the right way, although I will also use distilled water.

If she prepares an entry in the notebook, the first test of yellow liquid presumably insulin begins. The first bottle will have an amount of 20% yellow liquid, 10% distilled water and 10% alcohol. "

Sia, with her notebook, began to write that the first bottle had a certain concentration of liquids. When Einar had just put the yellow liquid in, he put the distilled water and the alcohol.

At the end the bottle was left with 20 ml of liquid, so I move on to the second bottle "This second bottle will have a concentration of 30% yellow liquid 15% distilled water and 15% alcohol."

Einar then began to fill the second vial and, in that way, he repeated the same procedure until he completely filled the ten vials that he had in front of him. "This batch of insulin vaccines has a concentration of 50% yellow liquid and 25% distilled water and 25% alcohol.

The same type of change is always maintained, only in different proportions in order to know which is the correct measure. To begin the test, we will start with the injections of the rabbit that extruded the pancreas. "

Einar then took a rabbit that was swollen because of problems caused by not having a pancreas or an effective way to digest.

"Subject 23 white rabbit with a weight of 1.58 kilograms without pancreas, according to the experiments with the previous subjects in 4 days, will die due to the lack of pancreas.

Alright little guy, let's get started on your insulin shot from batch D. "

Einar then took his glass syringe and took the substance in bottle 1, very carefully injected it into the animal and then turned an hourglass "The substance in bottle 1 was injected correctly into the subject 23 20% yellow liquid, 10% distilled water and 10% alcohol.

The mouse does not seem to have any improvement in the short term; I proceed to deliver a little carrot with honey so that little subject 23 can be fed. "

Einar then took a piece of white carrot and put a little honey on it, which he gave to the rabbit, who quickly began to eat the carrot.

"Very good little subject 23, you are doing very well, you have to survive because the life of my little daughter is at stake, she needs the valuable insulin so that she can live in peace, at least with that disease I can fight.

It would be impossible for me to fight cancer and I am not going to lose anyone else if I can do something to prevent the death of my family. "

Sia, who was already used to the nonsensical comments that Einar made, just kept writing down in her notebook while she looked at the hourglass because she recorded in real time what the rabbit's state was.

At ten minutes, subject 23 screamed and was struck dead in the test area, causing Einar to hit the table.

"Unlike Lot C, the subject lived longer, so we have to do a test by increasing the distilled water and reducing the alcohol. The cure should be close to us.

It is just a matter of further testing. Sia places Subject 23 in the dangerous waste canister for the servants to take them to the morgue oven to burn them, which are a health hazard. "

Einar sighed and threw out the entire batch D of bottles because it would be the same result. I check it from the test with batch A and B.

With new bottles he started the test again, for that he took a pig pancreas from a cooler with snow and with a syringe he began to obtain the yellow liquid that came out with a red substance that looked like blood.

When he finishes taking the liquid, he is in charge of throwing the red substance and he looks at the new bottles

"Bottle number 1, 20% yellow liquid, 15% distilled water and 5% alcohol will be used, leaving at the end a substance of 20ml."

Einar then placed the substances in the bottle and repeated the same process in the other 10 bottles. When he finished, he took from the cage a small rabbit that had the number 24 written on a necklace.

"Very well, subject 24, it is a rabbit without pancreas weighing 1.68kg,

Well, bunny, you will be injected with an injection of insulin from batch E, to test if with more distilled water and less alcohol, the substance stops being dangerous. It is expected that it will barely live about three days without the pancreas.

You have to survive, little one; I am not asking you for something impossible, just resist the first reaction and lead me to the path of success that my daughter's life is in danger. "

Einar then took the glass syringe and took the substance from bottle one, which he injected into the body of subject 24. Doing the same procedure gave the mouse a little white carrot with honey.

With the hourglass running, the minutes passed and there was sweat on Einar and Sia's foreheads as the hourglass was approaching 10 minutes, when the time indicated the death reaction did not happen.

Einar smiled and began to laugh because he could see time continue to run. After an hour, Einar took a thermometer and inserted it into the rabbit's rear to take the temperature.

At that moment, he realized that the rabbit was suffering an increase in temperature, something normal in the insulin that was not sufficiently purified. Einar was too excited that he hugged Sia as if she were his daughter.

"We did it, we managed to find a percentage of the vaccine that works, write it down in your notebook."

Sia also smiled and felt Einar's paternal affection, so when she stopped hugging her, she began to write in her notebook.

"On May 1, 832, the batch of vaccines E showed a functioning in the rabbit named subject 24, creating an adverse reaction in the organism in the form of fever.

This is caused by the impure insulin. Different grades of distilled water and yellow liquid should be tested. The alcohol for the moment remains in the 5% range, but if no improvement is shown in future subjects .

The concentration of alcohol should be increased, keeping it below 10% since the body does not seem to support that amount of alcohol in the blood, if a significant advance is found.

We have to start testing in different animals, from dogs to pigs, this to measure the amount of insulin needed according to weight and to be able to have an exact amount for an adult.

If the test is correct and there are no alternate reactions, it should be used in the Norma patient, with 24-hour supervision at all times, to know how many injections a day he will need. "

Einar rejoiced and patted the little rabbit who was suffering from a fever because of impure insulin in his organism.

"Little rabbit, unfortunately without your pancreas your life will come to the end in a few days but I will make a statue in the name of all the rabbits that died to obtain insulin, no one is going to die of diabetes in my nation.

Much less my daughter. I think that in the future I will be a rock star because they will say that I was a genius or that everything I did was for the help of aliens.

There are things that never change, but I suppose that at least I will leave my name in this era and people will remember me with love or hate, but that will only be for the students.

Especially for math, but not everyone could like it. "

Einar then turned his head and looked at Sia. "We can take an hour's break. I think we need to eat a little, because a balanced diet can help us think better.

But I think we can also celebrate a bit. We have created history this day because we found a non-lethal percentage of insulin; I know it may not seem as fabulous as the process of getting aluminum or electricity.

Although you have to admit that this procedure could save millions of lives in the future, of course it will require a stable income from pigs, but human life will always be a priority.

In addition, the pigs that die from the research will be well used to eat and produce food, which reminds me that today I will prepare to eat delicious pork ribs with honey, ketchup and pepper.

I'm sure you will love them. "

Sia nodded happily because if there was something she liked more than doing scientific research, it was eating the delicious food that Einar prepared. What she did with the food was fabulous.

Before arriving at Einar's laboratory, she only ate to satisfy her hunger, but now she can enjoy the food because its taste is fantastic. It is something that she can always remember, even if she is working.

After that, the two left the laboratory and started on their way to the kitchen of the imperial palace, leaving the rabbit in the laboratory who lost consciousness because of the fever he had.