Chapter 356

Taking the specimen from the human body, Lu Li walked out of the operating room.

When he came to a nearby test bench, Lu Li sat down and began to edit T cell genes.

Gene editing and modification, this technology has long appeared.

Gene modification by natural means, such as fruit tree grafting technology, and even hybrid rice technology, are all gene modification technologies.

People are also familiar with gene editing and modification by unnatural means, such as Transgenic technology.

Even if it's human genes, it's not once or twice.

As early as 2016, a guy named he Jiankui made a pair of so-called "immune AIDS" gene editing babies by manipulating human embryos, and then He Jiankui was put in jail!

It's so special to have a pair of gene editing babies when the technology is completely immature Is there any conscience and morality of scientists?

If gene technology is a thoroughfare, human beings have just taken a small step, and they want to play God? How stupid and arrogant is this?

The more advanced human science and technology are, the more awe we have to maintain.

Lu Li sat in front of the test bench, carefully took out a little sample of cancer cells, installed under the electron microscope.

In the image of electron microscope, the internal structure of cells is clearly displayed on the display screen.

Endoplasmic reticulum, ribosomes, Golgi apparatus, lysosomes, mitochondria, microsomes, vacuoles, cytoskeleton, cytosol, and even DNA entangled in the nucleus are clearly displayed.

DNA needs an electron microscope to see, but If you straighten it, the DNA chain is two meters long.

By manipulating the instrument, cas9 transcriptase was injected into cancer cells with a gene gun. Luli clearly saw that the transcriptase was close to the DNA chain and copied a piece of DNA information.

The gene information of cancer cells was transcribed.

Lu Li started his next experiment. The T cells are installed under the electron microscope, and the gene gun is used to inject the gene information of the cancer cells into the T cells.

Soon, T cells absorbed this genetic information and incorporated it into the "hostile sequence" of the immune system.

By doing so, weapons against cancer cells have been developed.

But Once injected into the human body, T cells will divide wildly, which will inevitably lead to the madness of the immune system and indiscriminate killing.

As a result, the immune system kills people before cancer kills them.

Therefore, Luli still has to carry out a step of operation.

This time, Luli is going to modify the "information dissemination mechanism" of T cells, so that it will no longer call for "air support" or "call for friendly forces" everywhere, but will only fight alone.

Perhaps, in this way, it may not cause the immune system to go crazy.

This involves another gene editing technique, crispr-cas9.

This gene editing technique is derived from the variation mechanism of bacteria against phages.

When bacteria resist phage attack, they will mutate a special protein, which can cut off the gene chain injected into the nucleus.

I have to mention that bacteriophages are the most terrible killers in the world.

Every moment, every corner of the world, there is a war that has lasted for countless years. The scale of the war has reached trillions of magnitude.

This is the phage attack on bacteria.

Phage is a kind of virus, its shape is very regular, the head is twenty dice, there is a small stick like trunk, below there are several spider legs, like a nano robot.

Bacteriophages are harmless to the human body. They just attack bacteria crazily. They inject their own DNA into bacteria and divide into numerous phages in cells, killing bacteria and spreading them.

In the face of phage attack, bacteria have evolved a means of resistance, which is a special protein mentioned above. The protein cuts off the DNA injected by the phage.

Human beings use this special protein to cut, modify and even edit genes.

In the laboratory, Lu Li repeated the experiment of bacteriophage attacking bacteria and obtained CRISPR, a special protein used by bacteria to fight against bacteriophage.

Then the CRISPR protein, combined with cas9 transcriptase, was injected into T cells to modify the genes of T cells.

Gene mapping is a problem.

Lu Li has done countless experiments in a row, but he has not been able to obtain accurate gene modification effect.

Even if the transcriptase matches the gene, however Shear proteins are often cut in the wrong places.

To manipulate genes is not to cut them with scissors. No tool can go deep into the nucleus, but to cut proteins.

Fortunately, transcriptase gene matching is still playing a role.After hundreds of repetitions, I finally got it right once.

A T cell, which has been genetically edited, was finally born.

Lu Li quickly got up and injected the T cell into the virtual human body.

Then it's waiting!

After gene editing, T cells enter the human body, immediately detect the existence of "hostile target" cancer cells, instantly activate the stress mechanism, begin to absorb nutrients from the human body, and divide crazily.

Half an hour later, the experimental body began to have a fever and the heart rate increased sharply.

It's the immune mechanism that works. The symptoms of fever are the normal mechanism by which the immune system responds to virus invasion.

And then The immune system is still crazy!

Even if T cells don't "call for friendly forces", the large army of T cells will still lead to a violent immune response in the human body, and the "factor storm" will still occur.

It's just It doesn't seem that big.

Lu Li quickly invented drugs to control the madness of the immune system and maintain the vital signs of the experimental human body.

Cardiotonic injection, adrenal hormone, first aid, and various emergency measures were used repeatedly. I don't know how long it took for this experimental human body to stabilize.

"Sure enough, my idea is right!"

Lu Li took a long breath and grinned, "eliminating the ability of T cells to call friendly forces can reduce the risk of immune system madness."

Although this little progress can not make a key breakthrough in car-t cancer treatment technology, but As a postgraduate thesis, the gold content is absolutely enough.

When he closed the virtual laboratory, Lu Li opened his eyes. Another night passed.

After getting up, washing up and going out for breakfast, Lu Li came home, sat in front of the computer and began to write his thesis.

"Car-t technology, screening T cell information transmission mechanism"

Lu Li sat in front of the computer desk, his hands crackling on the keyboard, a detailed data, clear experimental steps, real and reliable paper, easy to write.

One in Chinese, one in English, all done.

Then, Lu Li called Professor He, "teacher, my research project has been..."

"Have you thought about the subject? It's very efficient! "

Without waiting for Lu Li to finish, Professor He took over the conversation and said, "OK, you can send me the topic! I'll report it to you. "

"That It's not a topic, it's a paper. I wrote a paper

Lu Li quickly explained: "teacher, I wrote a paper and sent it to your email. Please correct it

"Thesis?"

Professor He Leng for a while, you did not even choose the topic, to write a paper? Is it the kind of irrigation paper with mixed names?

"I'll have a look!"

Professor he didn't say much. I'm going to read it first. If it is true, we should clean up Lu Li and kill this unhealthy trend.

Sitting back at the desk, professor he turns on the computer and sees the paper from Lu Li in the mailbox.

Click on the paper and see the title of "car-t technology, screening T cell information transmission mechanism", professor he was stunned.

Car-t technology? This is still a world leading scientific research project!

Information transmission mechanism of screen T cells? New gene editing technology?

Professor he suddenly looks a Su, quickly and seriously looked up.

The paper was in duplicate, one in Chinese and one in English. Professor he didn't bother to read the English version, so he picked up the Chinese version and read it.

There is no moisture in the whole paper, it's all dry goods.

In this paper, Lu Li described the new gene editing direction of T cells in detail, each step was clear, and the experimental data were also very detailed.

From the point of view of the paper, as long as we follow the experimental steps of land separation, this gene editing technology, which blocks the information transmission mechanism of T cells, is completely repeatable.

Theoretically, shielding the information transmission mechanism of T cells can effectively reduce the "factor storm" and reduce the probability of madness of the immune system.

"The boy Quietly, he let me have a bomb

Professor he had a bright smile on his face, "car-t technology breakthrough, this is a major scientific research achievement! pretty good! Good

After reading this paper, professor he quickly downloaded and printed it. Then, professor he picked up the papers and ran out.

All the way to Fudan Medical College cancer research room, found Professor Guo engaged in cancer medical technology research.

"Lao Guo, Lao Guo, take a look at this paper."

Professor he shouts at the same time and raises the information in his hand to Professor Guo.

"Thesis?"

Professor Guo of the cancer research office looked up at professor he and said, "Lao he, you are so excited. Is there something else in this paper?""More than goods?"

When he came to Professor Guo, he handed over his paper and said, "look at it for yourself!"

"Car-t technology, screening T cell information transmission mechanism? The author Lu Li

Seeing the title and the author, Professor Guo immediately became serious. "It turned out that Lu Li wrote it. This guy, did he study it? It's amazing

When he picked up the paper, Professor Guo sat down and read it carefully.

After reading it over and over again, Professor Guo's face lit up with a look of surprise. "From the perspective of experimental steps and ideas, this new direction of gene editing, unexpectedly Did it work? "

"It looks like that!"

Professor he grinned, "I came to you just to repeat this experiment."

"Yes

Professor Guo quickly got up, "I also want to verify it. If the experiment can be repeated, then That's great! "

It's amazing that a master's degree student has made such a breakthrough in cutting-edge scientific research projects with such a high gold content thesis.

Therefore, Professor Guo ordered all the master's and doctoral students in the cancer research laboratory to hand out a paper to them and ask them to repeat the experiment.

"Lu Li's thesis? Car-t technology

Seeing this paper, the doctoral students and master students in the research room are all dumbfounded.

It's less than a year since he was a graduate student, isn't it? That's the result of scientific research?

Originally, Lu Li's previous "consultation" made Professor Guo praise Lu Li all the time, which made everyone feel embarrassed.

Now You're so special that you've made a difference?

Does this make people live?

Secretly, these "research dogs" can only repeat Lu Li's experiment according to Professor Guo's instructions.

People immediately took action, just like the assembly line, constantly preparing shear protein, preparing transcriptase, and continuously injecting them into T cells.

Compared with Lu Li's one-man strategy of completing all the steps by himself, the current pipeline mode is much more efficient.

In an hour, more than 300 samples of T cell gene modification have been prepared in the laboratory.

Among the more than 300 samples, four T cells have achieved accurate screening of information transmission mechanism.

The experiment is successful!

"Ha ha ha! Sure enough, it's a success

Professor he couldn't help laughing and said, "Lu Li has really made achievements! Ha ha ha ha

Around the graduate students, doctoral students, one by one buried in silence.

Professor Guo looked at the successful preparation of T cells, and looked at Professor He, who couldn't close his mouth with laughter. He sighed in his heart.

How can such an excellent student fall into Lao he's hands? What luck!

Later, professor he published Lu Li's paper and submitted it to the core journal "Chinese medicine", and attached the repeated trial records of Fudan University.

After receiving this contribution, the editorial department of Chinese medical journal was shocked to see the title. When it saw the attached duplicate experiment record of Fudan University, it immediately transferred it to the reviewer without saying a word.

Fudan University has verified it once, which is clearly a scientific research achievement tested by practice.

There are several reviewers, some are medical professors of Yanjing University, some are academicians of Chinese Academy of Sciences.

After receiving the manuscript, the reviewers read it carefully.

After reading Lu Li's paper and seeing the repeated experiment record of Fudan University, the reviewers were amazed again.

Is the author a graduate student?

A master's degree student, actually made such scientific research achievements?

Is Fu Dan so powerful in scientific research?

With a sigh, the reviewer arranged another repeated experiment. As a result Once again, Lu Li's paper is absolutely correct.

Not long after that, the Journal of Chinese medicine officially published Lu Li's paper.

Once published, the paper has caused a huge shock in the scientific community, and also caused great repercussions in the society.

"Conquering cancer is no longer a dream. Lu Li, a Chinese scientist, has made a major breakthrough in car-t anti-cancer technology

Lu Li: is this a major breakthrough?

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